Intermediate

Part:BBa_K112341:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{rbs_pelB>} {< ligase>}{< phoA!}{< b1006>}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1396
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1396
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1396
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 1760
    Illegal NgoMIV site found at 2207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 420


Design Notes

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112341


Source

BBa_K112329 and BBa_K112324


References